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Phosphorylation of Ser307 in insulin receptor substrate-1 blocks interactions with the insulin receptor and inhibits insulin action

A more conservative escalation, extending each phase to six weeks instead of four, is a reasonable modification that some researchers adopt

10.1016/j.anireprosci.2019.106130 54

Subsequently, 10 L of the semen sample was pipetted onto a pre-warmed glass slide, gently covered with a coverslip, and the number of spermatozoa exhibiting coiled or swollen tails was counted under a microscope